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HEPA/UV Module

hepa-uv hero

Additional Documentation

For complete instructions on module installation and use, see the HEPA/UV Module Instruction Manual.

The top-mounted Opentrons Flex HEPA/UV Module is a clean air and ultraviolet (UV-C) disinfectant accessory for the Flex liquid handling robot. It contains a mesh pre-filter, a HEPA filter, and two UV lights. During operation, the HEPA system creates a positive pressure environment inside the Flex enclosure, which helps protect samples from contamination. When running the UV lights and air filtration for a full, 15-minute cycle, the HEPA/UV module can achieve ISO-5 clean bench standards within the Flex enclosure.

Warning

The HEPA/UV Module is not a biosafety cabinet. Do not use it with pathogens or to filter particles smaller than 0.3 micrometers (μm).

HEPA/UV Module features

Air filtration

The HEPA/UV Module relies on a 2-stage filtration system to purify air pulled into the enclosure. This system includes a reusable pre-filter and a disposable H14 HEPA main filter. The pre-filter traps large particles while the HEPA filter captures up to 99.99% of airborne particulate matter at ≥ 0.3 μm. Vertical air flow from the filter creates a positive pressure environment within the enclosure.

UV disinfection

The HEPA/UV Module uses two compact fluorescent bulbs that emit UV-C light at a wavelength of 254 nm. After a 15-minute exposure cycle, the UV light produced is sufficient to reach log-4 (99.99%) inactivation of commonly targeted microorganisms within the enclosure.

Safety features

The HEPA/UV Module includes safety features that protect you from UV-C exposure and prevent it from operating in an unsafe manner.

  • Panels: The robot's polycarbonate door and side panels block UV spectrum light to below a level that represents an exposure risk.

  • Door switch: Flex uses a mechanical switch to tell if the front door is open or closed. The UV lights only work when the front door is closed.

  • Attachment sensors: The Flex and HEPA/UV module each have a built-in sensor to detect if the module is attached properly. The sensors deactivate/disable the UV lights if the module is not mounted on the robot, removed while in operation, or misaligned.

HEPA/UV Module specifications

Specification Description
Dimensions 87 cm L x 64 cm W x 14 cm H
Weight ~20 kg (42 lbs)
Power input
  • 100–240 VAC, 50/60 Hz
  • 2.2 A at 115 VAC
  • 1.1 A 230 VAC
Power output 24 VDC, 8.4 A, 201 W max
Filter
  • Metal mesh pre-filter, reusable
  • H14 HEPA secondary filter, good for 3 years/6,000 hours, disposable
Ambient fan noise ≤ 70 dB at 1 meter during operation
Bulb type
  • Actinic fluorescent
  • 2G11 base (4 pin, single-cap)
Bulb life
  • 9,000 hours
  • UV end-of-life depreciation: 20%
Mercury (Hg) content 4.4 mg